Library · Biology & lab methods
What Chromatography & Protein Purification got wrong, from 70 theses
Summary written by a model from the records below: The records document numerous operational obstacles and failure mechanisms encountered across chromatographic purification workflows. Common difficulties include column fouling from non-specific adsorption, insufficient chromatographic resolution, complex disassembly, affinity tag failures, and unfavorable performance compared to simpler precipitation techniques. These records come from graduate theses, 2021 to 2026, each labeled with its degree where the repository states it, and each links to its thesis. They were extracted by language models reading the full text, so treat each as a lead to read, not a verdict.
Plus 9 problems the authors left open, listed at the end.
Failure modes, by theses reporting them
Select a mode to jump to its records.
Affinity purification fails due to tag inaccessibility, poor retention, or persistent host contaminant binding
Engineered affinity tags were frequently buried inside complexes or bound target matrices with insufficient affinity, leading to extensive protein loss during flow-through and wash steps. In addition, affinity resins frequently suffered from severe non-specific background binding and co-purification of host contaminants.
Tried and failed
recombinant bacterial expression with affinity chromatography applied to bacterial outer membrane proteins. Reason: protein aggregated into insoluble inclusion bodies and failed affinity column binding
The interaction of glycosylated major outer membrane protein of Campylobacter jejuni with natural and synthetic ligands · Nottingham · PhD
Tried and failed
affinity purification without heat-denaturation contaminant precipitation applied to sticky recombinant bacterial effector protein. Reason: protein stickiness caused co-purification of host contaminants and multiple gel filtration peaks
Structural and Functional characterisation of SteE, a Salmonella type III secretion system effector · Imperial · PhD
Tried and failed
immobilized metal affinity chromatography applied to recombinant bacterial protein purification. Reason: co-elution of an unexpected high molecular weight host protein impurity with the target
Cross-Species Analysis of YTH Domain Proteins from H. sapiens and A. ricinus · UT Austin
Tried and failed
His-tag affinity purification from membrane fractions applied to heterologously expressed bacterial membrane transporters. Outcome: no signal. Reason: Membrane extractions yielded excessive non-specific binding and failed to isolate the full-length target proteins.
The role of cyclic nucleotide-binding efflux pumps in Mycobacterium tuberculosis and antimicrobial resistance · Imperial · PhD
Tried and failed
immobilized metal affinity chromatography with cobalt resin applied to recombinant his-tagged membrane protein purification. Reason: weak His-tag binding caused almost total protein loss in flow-through and early wash steps
Investigation of the oligomeric state of the mycobacterial lipid transporter, MmpL3: biophysical and computational insights · Imperial · PhD
Tried and failed
immobilized metal affinity chromatography using imidazole gradient applied to recombinant histidine-tagged protein purification. Reason: leaky uninduced expression and persistent non-specific co-elution across imidazole concentrations
The Rtc RNA Repair System in E. coli: underlying molecular regulation and its role in infection and antibiotic resistance. · Imperial · PhD
Tried and failed
immobilized metal affinity chromatography for purification applied to secreted recombinant proteins in fermentation broth. Outcome: no signal. Reason: failed to detect or bind the tagged protein from the culture broth
Tried and failed
subtractive IMAC purification after affinity tag cleavage applied to recombinant protein purification. Reason: cleaved target protein retained unexpected intrinsic affinity for immobilized metal affinity chromatography resin
Analysis of the structure, function and protein-protein interactions of the popeye domain containing proteins · Imperial · PhD
Tried and failed
recombinant membrane protein purification using affinity tags applied to mitochondrial transporter expression in yeast. Reason: insufficient purified protein yield across various affinity tags, promoters, and culture media
Labeled nicotinamide adenine dinucleotide reveals the mechanism of mitochondrial NAD⁺ replenishment · UT Austin · PhD
Tried and failed
polyhistidine tag affinity purification and protease cleavage applied to membrane protein purification from mammalian cells. Reason: dominant host protein contamination and incomplete 3C protease cleavage within 24-48 hours
Structure of a silk moth fructose receptor: an ion channel involved in insect chemosensation · Harvard · PhD
Tried and failed
C-terminal epitope tagging for affinity purification applied to protein complex co-enrichment. Outcome: no signal. Reason: Enriched non-specific ribonuclear proteins instead of intended complex members
Investigation of E3 ligase ligands for targeted protein degradation · Harvard · PhD
Tried and failed
affinity purification using C-terminal affinity tag applied to multisubunit ribonucleoprotein complex lacking accessory subunit. Reason: the subunit tag interfered with assembly or complex was unstable without the missing subunit
Structures of Class 1 CRISPR-Cas surveillance complexes · UT Austin · PhD
Tried and failed
immobilized cobalt affinity chromatography purification applied to polyhistidine-tagged recombinant toxin protein. Reason: Weak target protein binding caused substantial loss in column flow-through and low imidazole wash fractions
Characterising the protein-protein interactions of TacTA1-3 toxin-antitoxin family · Imperial · Master's
Lost to a baseline
HisTrap HP purification produced higher gross yield (3 mg) but was contaminated compared to the lower yielding (100–282 µg) Strep-Tactin XT purification.
Structural and functional characterisation of the human vitamin c transporters · Imperial · PhD
Considered and rejected
Considered and rejected: Purification utilizing 6x His tags (both N- and C-terminal) on Ni/NTA resin due to excessive non-specific background binding.
Residual Neuropathy target esterase activity defines the PNPLA6 disorder spectrum · Johns Hopkins · PhD
Considered and rejected
Considered and rejected: Purification tags MBP and His6 for recombinant Mymx ectodomain due to failure to release protein without detergents
Myogenic Effectors and Disease · UT Southwestern · PhD
Considered and rejected
Considered and rejected: Rejected standard His-tag/Ni-NTA purification with imidazole elution due to interference with the catalytic non-heme iron center.
A study of prolyl hydroxylase domain 3 (PHD3): protein biochemistry and the development of inhibitors · Harvard · PhD
Considered and rejected
Considered and rejected: Rejected in vivo TruA cleavage during large-scale DAA680 purification because it cleaved the N-terminal His-tag needed for nickel affinity isolation
Considered and rejected
Considered and rejected: His-tag affinity purification of RNF168UDM1* was rejected due to low expression levels and protein instability, switching to an N-terminal GST-tag.
A chemical biology approach to decipher chromatin ubiquitylation by RNF168 · EPFL · PhD
Tried and failed
iminobiotin affinity chromatography purification applied to monoavidin surface-engineered extracellular vesicles. Reason: engineered monoavidin had insufficient binding affinity to iminobiotin resin at binding pH, leading to negligible recovery
Modular assembly of bacterial outer membrane vesicle-based vaccines and therapeutics · Cornell · PhD
Considered and rejected
Considered and rejected: Rejected Ni-NTA affinity chromatography for large-scale SMALP purification because target purity decreased with increasing scale, choosing Twin-Strep/Strep-Tactin instead
Biochemical Characterization of Glycan Assembly Pathway Enzymes · MIT · PhD
Considered and rejected
Considered and rejected: Rejected transferring standard affinity resin operating parameters directly to membrane chromatography without re-optimizing buffer ionic strength and pH due to severe unbinding/recovery failure.
Evaluating Improvements and Challenges in Affinity Chromatography for AAV Purification · Johns Hopkins · Master's
Considered and rejected
Considered and rejected: Discarded Strep-tag affinity chromatography for integrin purification because it diluted protein without increasing purity
Chromatographic resins fail to resolve species with similar hydrodynamic size, charge, or modification states
Separation matrices were unable to fractionate target molecules from closely related impurities, oligomers, or cleavage fragments possessing similar hydrodynamic properties or net charge. These resolution failures led to widespread co-elution across size exclusion, hydrophobic interaction, and ion exchange chromatography runs.
Tried and failed
size exclusion and density gradient centrifugation applied to protein purification of degradation products. Reason: failed to resolve impurities and degradation products with similar size or hydrodynamic properties
In vitro biochemical reconstitution and electron microscopy of early pre-DNA replication complex intermediates using human proteins · Imperial · PhD
Tried and failed
size-exclusion chromatography multimer fractionation applied to heterogeneous multi-domain protein complexes. Reason: columns failed to separate oligomeric species across different resins and construct variants
Structural and Biophysical Characterization of the Myocilin-OLF Domain Under Various Stressors · Georgia Tech · PhD
Tried and failed
size exclusion chromatography purification applied to micellar amphiphilic conjugates from liposomes. Reason: insufficient size difference to resolve free or micellar amphiphilic conjugate from PEGylated liposomes
Analysis of the properties of phospholipid bilayers for drug delivery using solid-state NMR · Imperial · PhD
Tried and failed
inclusion body prep with ion exchange chromatography applied to multiply modified recombinant proteins. Reason: standard chromatography steps lacked sufficient resolving power to yield pure modified target protein
Investigation of parameters of chromatin higher order structure and the making of multiply-acetylated histone H3 via nonsense suppression · Iowa State · PhD
Considered and rejected
Considered and rejected: Alumina column chromatography rejected for purifying amine-functionalized DAEs due to large particle size causing poor separation between product and impurities.
Design, Synthesis, and Structural Characterization of Photoswitchable Crystalline Materials · University at Buffalo · PhD
Tried and failed
anion exchange chromatography applied to protein-small molecule conjugate separation. Reason: insufficient charge differential caused co-elution of unmodified and modified protein
Modification of Biopolymers Using Palladium Oxidative Addition Complexes · MIT · PhD
Tried and failed
higher initial salt concentration in hydrophobic interaction chromatography applied to modified biopolymer purification. Outcome: worse than baseline. Reason: Excessive product retention delayed elution until high modifier concentrations and degraded chromatographic resolution.
Modification of Biopolymers Using Palladium Oxidative Addition Complexes · MIT · PhD
Tried and failed
single affinity pulldown and ion exchange chromatography applied to multi-subunit protein complex purification. Reason: failed to yield stoichiometric complex, causing non-stoichiometric subunit accumulation
A single-molecule approach to understanding sister chromatid cohesion establishment · Imperial · PhD
Tried and failed
affinity chromatography and size exclusion chromatography applied to fluorophore labeled membrane protein purification. Reason: host membrane proteins containing reactive cysteines co-purified and labeled alongside the target protein
Exploring the Structural Dynamics of Bacterial Chemotaxis · MIT · PhD
Tried and failed
ion-exchange chromatography for recombinant protein purification applied to recombinant bacterial beta-lactamase enzyme. Reason: produced heavily contaminated samples with minimal target protein yield
Tried and failed
elution using specific chaotropic displacing counterions applied to anion exchange chromatography of nucleic acids. Reason: Counterions caused either premature dead-volume elution or extreme retention and co-elution
Improving bioanalytical methods for nucleic acids with chemically tuned separations · Iowa State · PhD
Considered and rejected
Considered and rejected: Omitted ion exchange chromatography after IMAC as it failed to enrich singlet pentamers
Considered and rejected
Considered and rejected: Rejected protein A affinity chromatography and size-exclusion chromatography for separating WT Fab from Fc fragments post-papain digestion due to co-elution, switching to anion exchange chromatography at pH 8.
Site-specific conjugation for the synthesis of radiopharmaceuticals · Imperial · PhD
Non-specific stationary phase adsorption causes severe sample loss and column sticking
Target proteins and synthetic macromolecules suffered extensive or total recovery loss due to irreversible interactions with chromatographic resins. These non-specific binding and matrix sticking issues occurred primarily during size-exclusion and ion-exchange separation procedures.
Tried and failed
size exclusion chromatography purification applied to recombinant multiprotein complex. Reason: severe sample loss caused by non-specific matrix sticking and aggregation
In vitro biochemical reconstitution and electron microscopy of early pre-DNA replication complex intermediates using human proteins · Imperial · PhD
Tried and failed
size exclusion chromatography applied to purified multi-protein complex. Reason: hydrophobic interactions with the stationary phase matrix caused irreversible protein adsorption and sample loss
Characterization of the DPP8/9 Pyroptotic Checkpoints · Cornell · PhD
Tried and failed
size exclusion chromatography after ion exchange applied to multimeric protein complex purification. Reason: hydrophobic interactions with the column matrix caused complete sample loss
Molecular mechanisms for the regulation and assembly of the NLRP1 and CARD8 inflammasomes · Harvard · PhD
Tried and failed
membrane filtration and size exclusion chromatography applied to nanoparticle purification. Reason: severe particle agglomeration and material loss caused by filter adherence
Integrated Diamond-Based Devices For Quantum Sensing And Communication · Penn · PhD
Tried and failed
size exclusion chromatography with surfactant mobile phase applied to nanoparticle and organic matter mixtures. Reason: severe irreversible stationary phase adsorption and sample carryover
Investigation of zinc nanoparticles in soil environments · Nottingham · PhD
Tried and failed
size exclusion chromatography with standard organic columns applied to deprotected cationic polyesters. Reason: strong irreversible interactions between protonated amine groups and the polystyrene-divinylbenzene stationary phase
Multifunctional polyester-based stimuli-responsive polymeric nanoparticles for therapeutic delivery · Imperial · PhD
Considered and rejected
Considered and rejected: Rejected MWCO spin columns and Protein G beads for barcoded antibody purification in favor of ammonium sulfate precipitation due to lower yield (37-55%) and column sticking.
Development of Novel Tools for Highly Multiplexed Proteomics and Analysis of Transcriptional Circuitry · Johns Hopkins · PhD
Considered and rejected
Considered and rejected: Rejected standard size-exclusion chromatography for NLRP1-DPP9 complex purification due to complete sample loss from hydrophobic column interactions, relying instead on Mono Q anion exchange
Characterization of the DPP8/9 Pyroptotic Checkpoints · Cornell · PhD
Considered and rejected
Considered and rejected: Anion exchange chromatography (AEX) was omitted for His6-TEV-GGG-16E7 purification due to excessive protein loss.
Cellular and Intraviral Interaction Partners of Papillomavirus E6 Protein · Tübingen · PhD
Considered and rejected
Considered and rejected: Rejected size exclusion chromatography (SEC) for large-scale purification of CD630_08730 because it caused unacceptable loss of target protein yield.
Development of an oral vaccine against Clostridioides difficile · Nottingham · PhD
Considered and rejected
Considered and rejected: Rejected size exclusion chromatography (gel filtration) after affinity purification because recovery yield dropped to ~10% without fully removing degradation products.
Study of glyceraldehyde-3-phosphate oxidoreductases from archaea and bacteria · Nottingham · PhD
Considered and rejected
Considered and rejected: Rejected DEAE-C anion exchange chromatography for peptide purification due to substantial loss of peptide recovery, replacing it with cold 1:1 Et2O:hexane precipitation before HPLC.
Biosynthetic and Chemical Investigation of Lipid II-Binding Antimicrobials. · Duke · PhD
Lost to a baseline
Protein G purification resulted in significantly lower intact protein yield (62.5% loss for KbIg, 67.0% for A2Ig) compared to standard Sepharose-NP/NIP extraction (26% loss for KbIg, 44.9% for A2Ig)
OPTIMIZATION OF METHODS FOR MHC-IG SYNTHESIS, PURIFICATION, AND EVALUATION FOR T CELL IMMUNOENGINEERING · Johns Hopkins · Master's
Protein complexes dissociate or aggregate during chromatographic separation steps
Weakly associated multi-protein complexes and destabilized target proteins frequently dissociated into constituent subunits or formed insoluble aggregates during chromatography. Changes in buffer ionic strength or prolonged residence times on columns triggered protein misfolding, void volume elution, and loss of biological activity.
Tried and failed
recombinant full-length protein expression and purification applied to bacterial effector protein SteC. Outcome: unstable. Reason: severe protein aggregation causing elution in size exclusion chromatography void volume
Studying the mechanism of Salmonella effector protein, SteC · Imperial · PhD
Tried and failed
size-exclusion chromatography mass spectrometry for complex isolation applied to weakly binding monomeric peptide-protein complexes. Outcome: no signal. Reason: weak binding affinity caused complex dissociation during column separation
Affinity Maturation of Peptides to Bind Protein-Protein Interfaces · MIT · PhD
Tried and failed
size-exclusion chromatography co-elution of purified membrane proteins applied to mutated outer membrane protein complexes. Outcome: unstable. Reason: insertion mutations prevented stable in vitro complex formation between the interaction partners
The structural basis of F-like plasmid conjugation and surface exclusion · Imperial · PhD
Tried and failed
size exclusion chromatography in low-cosolvent buffer applied to destabilized recombinant kinase domain variants. Outcome: unstable. Reason: insufficient glycerol concentration failed to prevent protein aggregation and void volume elution
Biochemical characterization of EGFR exon 20 insertion variants and their inhibitor sensitivities · Harvard · PhD
Tried and failed
recombinant protein co-expression for complex purification applied to heteromeric protein complex assembly. Outcome: unstable. Reason: complex dissociated during size-exclusion chromatography
Structural and biochemical studies of RAD51 filament modulators · Imperial · PhD
Tried and failed
co-purification via affinity and size-exclusion chromatography applied to multimeric ribonucleoprotein-associated effector complexes. Reason: effector subunit lacked sufficient binding affinity to remain stably associated during chromatography steps
Structures of Class 1 CRISPR-Cas surveillance complexes · UT Austin · PhD
Considered and rejected
Considered and rejected: Abandoned co-expression and size-exclusion chromatography strategies for FIGNL1-RAD51 complex purification due to resin interaction and complex dissociation
Structural and biochemical studies of RAD51 filament modulators · Imperial · PhD
Tried and failed
native protein purification with affinity tags applied to intrinsically disordered protein purification. Outcome: unstable. Reason: extensive protein degradation and aggregation occurred under non-denaturing conditions
THE NEUROSPORA CRASSA CIRCADIAN CLOCK: AN ORDERLY LOOP DRIVEN BY A DISORDERED CONDUCTOR · Cornell · PhD
Tried and failed
his-tag affinity purification without heat denaturation applied to self-assembling fusion proteins. Reason: uncontrolled oligomerization caused protein smearing during electrophoretic separation
Tried and failed
size-exclusion chromatography under varying ion concentrations applied to measuring ion-dependent protein trimerization threshold. Outcome: unstable. Reason: protein instability and monomer unfolding occurred at low ion concentrations
Structural and biochemical investigations of collagen-I trimerization · MIT · PhD
Considered and rejected
Considered and rejected: Rejected ion exchange chromatography in low salt buffers for hMiro1 and hMiro1(R450C) due to heavy protein precipitation
Structural Analysis of Protein Complexes · Tübingen · PhD
Considered and rejected
Considered and rejected: Rejected size exclusion chromatography at room temperature for rDps purification due to protein cleavage/instability, switching to gradient pH elution on Ni-NTA resin.
Identification and characterisation of of vaccine antigens for Klebsiella pneumoniae · University College Dublin · PhD
Multi-step chromatography is outperformed or replaced by bulk precipitation and extraction baselines
Column chromatography procedures were frequently abandoned in favor of crude precipitation, liquid extraction, or simpler gravity methods. Researchers preferred these alternative techniques because column methods suffered from lower product yields, longer run times, and prohibitive processing costs.
Lost to a baseline
qEV size-exclusion chromatography resulted in lower RNA recovery and higher Ct values compared to ultracentrifugation, PEG precipitation (ExoQuick), and membrane affinity (exoEasy).
Evaluation of Cell-Free RNA as Minimally Invasive Biomarkers of Target Engagement · Harvard · Master's
Lost to a baseline
Size-exclusion chromatography (SEC) on Sephadex LH-20 with aqueous methanol required a 2- to 3-fold increase in run time (24 h vs 30-60 min flash chromatography) and suffered from low loading capacity (few hundred mg) and loss of stationary phase during packing cycles.
Microcystins as Lead Structures for Anticancer Agents · Tübingen · PhD
Lost to a baseline
Affinity chromatography using a CPV-coupled Sepharose column resulted in high sample loss and lower yields compared to mixed-mode ceramic hydroxyapatite purification
UNDERSTANDING IMMUNITY IN DOGS: STUDIES OF ATOPIC DERMATITIS AND THE ANTIBODY REPERTOIRE TO PARVOVIRUSES · Cornell · PhD
Tried and failed
sequential purification before covalent bioconjugation applied to heterobifunctional protein assembly. Outcome: worse than baseline. Reason: isolated intermediate purification steps decreased overall product recovery and increased processing time versus one-pot assembly
Engineering Tools to Promote and Characterize Wnt-Mediated Stem Cell Differentiation · Georgia Tech · PhD
Lost to a baseline
HisTrap affinity and Superdex 75 Increase SEC columns gave lower yields than simple gravity flow Ni-NTA purification (>10 mg/L culture, ~1 mM)
Structure and interactions of endonuclease in the influenza a polymerase complex · Oxford · Master's
Considered and rejected
Considered and rejected: Rejected Protein A chromatography as the primary method for large-scale Pep12-Fc purification due to its slow required flow rate, choosing ammonium sulfate precipitation instead
Inhibition of Essential Protein-Protein Interactions in Plasmodium and SARS-CoV-2 · Johns Hopkins · Master's
Considered and rejected
Considered and rejected: Rejected Melon Gel IgG Purification in favor of ammonium sulfate precipitation because ammonium sulfate was equally effective but more cost-effective and less time-intensive.
Nachweis funktioneller Autoantikörper gegen den Angiotensin-II-Typ-1-Rezeptor bei Patienten mit Systemischer Sklerose · Tübingen · PhD
Considered and rejected
Considered and rejected: Rejected chromatographic multi-step enzyme purification for environmental application due to prohibitive downstream processing costs, choosing crude ammonium sulfate precipitation.
Cold Active Enzyme Booster Technology (EnBooT) for Biodegradation of P-Xylene · York University · PhD
Considered and rejected
Considered and rejected: Discarded purification of tag-free σA via DEAE, MonoQ, Heparin, or size exclusion chromatography due to insufficient protein yield and purity.
Funktion und Metabolismus der regulatorischen, nicht-kodierenden 6S RNAs aus Bacillus subtilis Function and metabolism of the regulatory non-coding 6S RNAs of Bacillus subtilis · University of Marburg · PhD
Extreme analyte polarity and solvent incompatibilities hinder liquid chromatography
Target molecules exhibiting extreme hydrophobicity were insoluble in standard liquid chromatography mobile phases or aggregated upon dissolution attempts. Conversely, highly hydrophilic or acid-sensitive analytes failed to retain on reverse-phase media or decomposed during post-separation processing.
Tried and failed
size exclusion chromatography molecular weight characterisation applied to poorly soluble rigid polymers. Reason: polymer was only soluble in solvents incompatible with standard chromatographic equipment
Water vapor and CO₂ sorption and transport in carbon molecular sieve membranes and moisture swing materials · UT Austin · PhD
Considered and rejected
Considered and rejected: Rejected inverse-size exclusion chromatography (ISEC) using spherical proteins (ovalbumin, haemoglobin) because misfolding/aggregation under varying mobile phase compositions caused unreliable results.
Fundamentals of peptide adsorption in reversed-phase liquid chromatography · Imperial · PhD
Tried and failed
solid-phase peptide synthesis and chromatographic purification applied to hydrophobic dipeptide repeat proteins. Reason: extreme insolubility and aggregation prevented purification despite successful chemical synthesis
The Discovery and Chemical Synthesis of Peptides and Proteins that Cross Biological Barriers · MIT · PhD
Tried and failed
solution-phase purification and characterization applied to highly hydrophobic oligopeptoids. Reason: extreme hydrophobicity prevented dissolution required for liquid-phase purification and characterization
PRECISION ENGINEERING OF SMALL MOLECULES AND OLIGOMERS FOR ADVANCED TECHNOLOGIES · Cornell · PhD
Considered and rejected
Considered and rejected: High-performance liquid chromatography-mass spectrometry (HPLC-MS) was rejected for CRP1 analysis because CRP1 is highly hydrophilic/charged and does not retain on reverse-phase HPLC columns.
Searching For Metallophores And Metal Reactive Metabolites in Candida albicans · Johns Hopkins · Master's
Considered and rejected
Considered and rejected: Abandoned RP-HPLC purification of free C-terminal azidated peptides (CBz-Pro(N3)-OH) due to decomposition during lyophilization in acidic conditions
Azide-Containing Hypervalent Iodine Reagents for Biomolecules Functionalization · EPFL · PhD
Left open by the authors
Problems the authors named and did not get to.
Left open
Demonstrate direct metal transfer or stable complex formation between ZagA and FolE using size exclusion chromatography in the presence of ZTP. Blocker: Requires wet lab biochemistry facilities, purified proteins (ZagA, FolE), ZTP, and size exclusion chromatography instrumentation.
METAL TRAFFICKING DURING LIMITATION AND EXCESS IN BACILLUS SUBTILIS · Cornell · PhD
Left open
Optimize sample preparation using size-exclusion chromatography and collect cryo-EM particle data to reconstruct the DNA mismatch repair initiation complex in RELION. Blocker: Requires wet lab facilities for protein purification, chromatography, and access to a cryo-electron microscope
The Crosstalk Between DNA Mismatch Repair and Replication · UT Southwestern · PhD
Left open
Evaluate enhanced coagulation on isolated low and high molecular weight wastewater fractions to determine size-specific removal mechanisms. Blocker: Requires a wet chemistry laboratory, wastewater samples, fractionation membranes, and coagulation experimental apparatus.
Enhanced Coagulation Process for Dissolved Organic Nitrogen (DON) Removal from Wastewater · University of Nevada, Reno · PhD
Left open
Characterize intermediate fractions between cell debris and extracellular vesicle sizes, and define extracellular vesicle compositions from MSC lysates. Blocker: Requires wet lab equipment, cell cultures, and biological fractionation/characterization assays.
The immunoregulation of dying cell components on macrophages and their therapeutic potential in ischemic muscle regeneration · UT Austin · PhD
Left open
Implement cation exchange chromatography targeting N- and C-terminal globular domains for recombinant lubricin purification to prevent loss during high-salt wash. Blocker: Requires a wet lab and physical chromatography equipment/protein samples
LUBRICIN IN POST-TRAUMATIC OSTEOARTHRITIS, FROM NATURALLY OCCURRING DISEASE TO RECOMBINANT THERAPEUTICS · Cornell · PhD
Left open
Develop an anion ion chromatography method to separate and quantify polythionates for tracking sulfite reservation in amine scrubbing campaigns. Blocker: Requires a physical analytical chemistry wet lab and an ion chromatography instrument with chemical standards.
Understanding and mitigating amine oxidation caused by NO₂ in scrubbing to capture CO₂ · UT Austin · PhD
Left open
Develop an extraction and secondary chromatographic method using polar sorbents or anion exchange resins to retain short-chain PFAS analytes. Blocker: Requires an analytical chemistry wet lab, chromatography equipment, and chemical standards.
Evaluating "Forever Chemicals" from Soup to Nuts: Sample Preparation, Targeted Analysis, and Suspect Screening for the Characterization of Per- and Polyfluoroalkyl Substances (PFAS · University at Buffalo · PhD
Left open
Test shaking instead of sonication during extraction of PFAS from aluminum foil cascade impactor filters to prevent foil disintegration. Blocker: Requires a wet chemistry analytical lab, chemical reagents, and chromatography/mass spectrometry equipment (GC-MS, UPLC-MS)
Per and Polyfluoroalkyl Substances (PFAS) in Indoor Airborne Particulate Matter · Carleton University · Master's
Left open
Synthesize and test phosphonium, pyridinium, and ammonium ionic liquids with non-Tf2N anions using inverse gas chromatography to measure separation selectivity. Blocker: Requires a wet chemistry lab, chemical synthesis of ionic liquids, and gas chromatography apparatus.
Inverse gas chromatography for crucial separations using ionic liquids · UT Austin · PhD
Who has worked on this
Authors of the public theses cited on this page, most-cited first.
- Alexander Carver · Imperial · PhD · 2023
Structural and biochemical studies of RAD51 filament modulators - Shenaz Allyjaun · Imperial · PhD · 2023
In vitro biochemical reconstitution and electron microscopy of early pre-DNA replication complex intermediates using human proteins - Jacob J. L. Rodriguez · MIT · PhD · 2023
Modification of Biopolymers Using Palladium Oxidative Addition Complexes - Aleksandra Stasiak · Tübingen · PhD · 2023
Structural Studies of N-Acetylmuramic Acid Kinases from the Bacterium Tannerella forsythia and of the Human Adenovirus Penton Base-Integrin Entry Receptor Complex - Andrew Griswold · Cornell · PhD · 2021
Characterization of the DPP8/9 Pyroptotic Checkpoints - Mark Yao · Harvard · Master's · 2026
Evaluation of Cell-Free RNA as Minimally Invasive Biomarkers of Target Engagement - Farah Kabir · Harvard · PhD · 2025
Investigation of E3 ligase ligands for targeted protein degradation - Sorin Asha Srinivasa · MIT · PhD · 2025
Structural and biochemical investigations of collagen-I trimerization - Hailee Scelsi · Georgia Tech · PhD · 2025
Structural and Biophysical Characterization of the Myocilin-OLF Domain Under Various Stressors - Brandon Michael Gorodokin · Johns Hopkins · Master's · 2025
OPTIMIZATION OF METHODS FOR MHC-IG SYNTHESIS, PURIFICATION, AND EVALUATION FOR T CELL IMMUNOENGINEERING - Yueran Hou · University College Dublin · PhD · 2025
Identification and characterisation of of vaccine antigens for Klebsiella pneumoniae - I Rhyu · Oxford · Master's · 2025
Structure and interactions of endonuclease in the influenza a polymerase complex
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